Cell Imaging

ASB – Aline Wilmot Skaggs Biology Building: map

Number: 82
Code: ASB
Address: 259 S 1400 E Salt Lake City, UT 84112

CSC – Crocker Science Center: map

Number: 5
Code: CSC
Address: 1390 Presidents Cir Salt Lake City, UT 84112

EEJMRB – E.E. Jones Medical Research: map

Number: 565
Code: EEJMRB
Address: 15 N Medical Dr East Salt Lake City, UT 84112

HCI – Huntsman Cancer Institute: map

Number: 555
Code: HCI
Address: 2000 Circle of Hope Salt Lake City, UT 84103

HSC – Health Science Center: map

Number: 585
Code: RB LAB
Address: 30 N 2030 E Salt Lake City, UT 84112

SMBB – Sorenson Molecular Biotechnology (USTAR): map

Number: 151
Code: SMBB
Address: 36 S Wasatch Dr Salt Lake City, UT 84112

To Set Up a User Account:

Account SetupBooking Calendar:

1. Fill out and submit a Work Authorization form.  Work Authorization Form.
   • If you need help navigating the form, a ‘How to’ guide is available under the drop down menu labeled ‘Work Auth Forms
   • For additional questions please contact the administrative office at billing@cores.utah.edu
2. After your authorization is approved, contact us at our email support.cellimaging@cores.utah.edu
3. Training and an initial project consultation is required and helps us ensure you receive the best images possible that your samples produce.
   • Through our email contact we an give you suggestions on which of our staff bests fits your experiment and goals for training and assistance
   • additional training information is located under the ‘Training Tab’.
4. Booking on the calendar website
   • Login using your unid (ex. uxxxxxx) and cis password.
   • After logging in, the Cell Imaging Core calendar is located under ‘Resources’ and ‘Cell Imaging’
   • Each Staff member and microscope has a calendar that shows availability that you may book time for your use. Our HSC, HCI and Crocker locations are staffed from 9am-1pm Monday through Friday. Reservations for training and consulations at a specific location (e.g. Morning HCI consolation/training will be with whomever is staffed at that location). If you want an appointment with a specific staff member, choose appointments in the afternoon and the location just like before. For any questions regarding our new calendar system please email us at support.cellimaging@cores.utah.edu.
   • After booking staff member’s time, check the drop down option next to ‘Process’ to add location. Under ‘Data Fields’ and ‘Label add a description of intended use and experiment type.
5. Microscope
   • First Step: Book time slot with suggested staff for a one-hour mandatory start up project consultation. There you will review goals and plan.
   • Second Step: Book time with both the staff member and suggested microscope simultaneously for initial hardware and software training.
   • Third step: *Optional* After initial training it is suggested to book time with staff for first imaging session.
6. Building access granted after full training is complete
   • HCI
     • Computer and building access forms will be provided
     • Submit forms per our instructions
   • HSC and CSC
     • You must then go in person with your UID card to our Administrative Office to gain card access to the building and rooms to which you have been granted permissions. To get to our Administrative Office (Skaggs Bldg 582, Room 250), enter from the South doors, go up the stairs to the second floor, the offices are straight down the hall.
   • SMBB
     • We will send your information to the SMBB building office.

If you have any more questions concerning this process please contact us at support.cellimaging@cores.utah.edu.

Calendar bookings must be deleted by the user 24 hours prior to the time reserved in order to avoid billing charges. Booking time cannot be altered or deleted and is non-refundable nor creditable if this time window is not met. The Cell Imaging Core is not liable if a user no longer needs their booking, if a user’s samples do not work, if a user cannot make their booking, etc. However, if an instrument is down, is offline, or is under maintenance all fees will be voided for that duration.

Users with overages or caught with unregistered time can be charged the commercial rate of $100/h.

There are two tracks of training offered by the Cell Imaging Core

1. Short time users: Patrons that plan on 20 hours or less of usage per year.
   1. Assisted Imaging
      1. Staff will image your samples exclusively with you. Your presence is required for an assessment consultation of your sample quality and image goals.
      2. Signature of a release form is required to insure the quality of samples and the resulting images.
      3. Acknowledgement in publications for the University of Utah Cell Imaging is a requirement for this type of extensive assistance.

2. Long time users: Patrons that prefer in depth training for skill development with the goal of imaging yourself and to obtain rigorous understanding of software.
   1. Advanced training series
      1. Project consultation and basic training
      2. Advanced training for special software implementation (FRET, FRAP, live cell imaging, stitching, time lapse, ect.. )

Publications

2021

1. Ahmed, S. M. H., J. A. Maldera, D. Krunic, G. O. Paiva-Silva, C. Penalva, A. A. Teleman and B. A. Edgar (2020). “Fitness trade-offs incurred by ovary-to-gut steroid signalling in Drosophila.” Nature 584(7821): 415-419. DOI: 10.1038/s41586-020-2462-y.
2. Bell, L. A., G. J. Wallis and K. S. Wilcox (2020). “Reactivity and increased proliferation of NG2 cells following central nervous system infection with Theiler’s murine encephalomyelitis virus.” J Neuroinflammation 17(1): 369. DOI: 10.1186/s12974-020-02043-5.
3. Bhatlekar, S., B. K. Manne, I. Basak, L. C. Edelstein, E. Tugolukova, M. L. Stoller, M. J. Cody, S. C. Morley, S. Nagalla, A. S. Weyrich, J. W. Rowley, R. M. O’Connell, M. T. Rondina, R. A. Campbell and P. F. Bray (2020). “miR-125a-5p regulates megakaryocyte proplatelet formation via the actin-bundling protein Lplastin.” Blood 136(15): 1760-1772. DOI: 10.1182/blood.2020005230.
4. Davis, B., S. Erickson, S. Wojtalewicz, A. Simpson, C. Metcalf, H. Sant, J. Shea, B. Gale and J. Agarwal (2020). “Entrapping bupivacaine-loaded emulsions in a crosslinked-hydrogel increases anesthetic effect and duration in a rat sciatic nerve block model.” Int J Pharm 588: 119703. DOI: 10.1016/j.ijpharm.2020.119703.
5. de Hart, N., Z. S. Mahmassani, P. T. Reidy, J. J. Kelley, A. I. McKenzie, J. J. Petrocelli, M. J. Bridge, L. M. Baird, E. D. Bastian, L. S. Ward, M. T. Howard and M. J. Drummond (2021). “Acute Effects of Cheddar Cheese Consumption on Circulating Amino Acids and Human Skeletal Muscle.” Nutrients 13(2). DOI:
10.3390/nu13020614.
6. Derksen, A., H.-Y. Shih, D. Forget, L. Darbelli, L. T. Tran, C. Poitras, K. Guerrero, S. Tharun, F. S. Alkuraya, W. I. Kurdi, C.-T. E. Nguyen, A.-M. Laberge, Y. Si, M.-S. Gauthier, J. L. Bonkowsky, B. Coulombe and G. Bernard (2021). “Variants in LSM7 impair LSM complexes assembly, neurodevelopment in zebrafish and
may be associated with an ultra-rare neurological disease.” Human Genetics and Genomics Advances 2(3): 100034. DOI: https://doi.org/10.1016/j.xhgg.2021.100034.
7. Ferrara, P. J., X. Rong, J. A. Maschek, A. R. Verkerke, P. Siripoksup, H. Song, T. D. Green, K. C. Krishnan, J. M. Johnson, J. Turk, J. A. Houmard, A. J. Lusis, M. J. Drummond, J. M. McClung, J. E. Cox, S. R. Shaikh, P. Tontonoz, W. L. Holland and K. Funai (2021). “Lysophospholipid acylation modulates plasma membrane lipid organization and insulin sensitivity in skeletal muscle.” J Clin Invest 131(8). DOI: 10.1172/JCI135963.
8. Ferrari, L. F., J. Pei, M. Zickella, C. Rey, J. Zickella, A. Ramirez and N. E. Taylor (2021). “D2 Receptors in the Periaqueductal Gray/Dorsal Raphe Modulate Peripheral Inflammatory Hyperalgesia via the Rostral Ventral Medulla.” Neuroscience 463: 159-173. DOI: 10.1016/j.neuroscience.2021.03.035.
9. Keefe, M. D., H. E. Soderholm, H. Y. Shih, T. J. Stevenson, K. A. Glaittli, D. M. Bowles, E. Scholl, S. Colby, S. Merchant, E. W. Hsu and J. L. Bonkowsky (2020). “Vanishing white matter disease expression of truncated EIF2B5 activates induced stress response.” Elife 9. DOI: 10.7554/eLife.56319.
10. Lewis, M. R., Edgar, B. A., & ØVrebø, J. I. (2021). SIGNALING PATHWAY REGULATION OF TRANSCRIPTION FACTOR E2F1 ALTERS CELL CYCLE PROGRESSION IN DROSOPHILA MIDGUT.SIGNALING PATHWAY REGULATION OF TRANSCRIPTION FACTOR E2F1 ALTERS CELL CYCLE
PROGRESSION IN DROSOPHILA MIDGUT, 21(2021). https://d2vxd53ymoe6ju.cloudfront.net/wpcontent/uploads/sites/19/2021/05/21165945/LEWIS_MEGAN.pdf
11. Li, J., B. D. Mahoney, M. S. Jacob and S. J. C. Caron (2020). “Visual Input into the Drosophila melanogaster Mushroom Body.” Cell Rep 32(11): 108138. DOI: 10.1016/j.celrep.2020.108138.
12. Lyu, Y., V. K. Verma, Y. Lee, I. Taleb, R. Badolia, T. S. Shankar, C. P. Kyriakopoulos, C. H. Selzman, W. Caine, R. Alharethi, S. Navankasattusas, T. Seidel, S. G. Drakos and F. B. Sachse (2021). “Remodeling of tsystem and proteins underlying excitation-contraction coupling in aging versus failing human heart.” NPJ Aging Mech Dis 7(1): 16. DOI: 10.1038/s41514-021-00066-7.

2020

1. Deshpande, I., Liang, J., Hedeen, D., Roberts, K. J., Zhang, Y., Ha, B., Manglik, A. (2019). Smoothened stimulation by membrane sterols drives Hedgehog pathway activity. Nature, 571(7764), 284-288. doi:10.1038/s41586-019-1355-4
2. Dong, Z. M., Lin, E., Wechsler, M. E., Weller, P. F., Klion, A. D., Bochner, B. S., Clayton, F. (2020). Pulmonary Eosinophilic Granulomatosis with Polyangiitis Has IgG4 Plasma Cells and Immunoregulatory Features. Am J Pathol, 190(7), 1438-1448. doi:10.1016/j.ajpath.2020.03.005
3. Feng, H., Hockin, M., Zhang, S., Capecchi, M., Gale, B., & Sant, H. (2020). Enhanced chromosome extraction from cells using a pinched flow microfluidic device. Biomed Microdevices, 22(2), 25. doi:10.1007/s10544-020-0477-7
4. Feng, H., Magda, J. J., & Gale, B. K. (2019). Viscoelastic second normal stress difference dominated multiplestream particle focusing in microfluidic channels. Appl Phys Lett, 115(26), 263702. doi:10.1063/1.5129281
5. Gandelman, M., Dansithong, W., Figueroa, K. P., Paul, S., Scoles, D. R., & Pulst, S. M. (2020). Staufen 1 amplifies proapoptotic activation of the unfolded protein response. Cell Death Differ. doi:10.1038/s41418-020-0553-9
6. Guo, P., Nalder, S.-a., Okada, M., & Sigala, P. A. (2020). Doxycycline has Distinct Apicoplast-Specific Mechanisms of Antimalarial Activity. bioRxiv, 2020.2006.2011.146407. doi:10.1101/2020.06.11.146407
7. Hanak, T. J., Libbey, J. E., Doty, D. J., Sim, J. T., DePaula-Silva, A. B., & Fujinami, R. S. (2019). Positive modulation of mGluR5 attenuates seizures and reduces TNF-alpha(+) macrophages and microglia in the brain in a murine model of virus-induced temporal lobe epilepsy. Exp Neurol, 311, 194-204.
doi:10.1016/j.expneurol.2018.10.006
8. Hoffman, L. M., Smith, M. A., Jensen, C. C., Yoshigi, M., Blankman, E., Ullman, K. S., & Beckerle, M. C. (2020). Mechanical stress triggers nuclear remodeling and the formation of transmembrane actin nuclear lines with associated nuclear pore complexes. Mol Biol Cell, 31(16), 1774-1787. doi:10.1091/mbc.E19-01-0027
9. Kim, H. S., Neugebauer, J., McKnite, A., Tilak, A., & Christian, J. L. (2019). BMP7 functions predominantly as a heterodimer with BMP2 or BMP4 during mammalian embryogenesis. Elife, 8. doi:10.7554/eLife.48872
10. Lim, K., Sima, M., Stewart, R. J., & Minteer, S. D. (2020). Direct bioelectrocatalysis by redox enzymes immobilized in electrostatically condensed oppositely charged polyelectrolyte electrode coatings. Analyst, 145(4), 1250-1257. doi:10.1039/c9an02168j
11. Mahmassani, Z. S., Reidy, P. T., McKenzie, A. I., Petrocelli, J. J., Matthews, O., de Hart, N. M., Drummond, M. J. (2020). Absence of MyD88 from Skeletal Muscle Protects Female Mice from Inactivity-Induced Adiposity and Insulin Resistance. Obesity (Silver Spring), 28(4), 772-782. doi:10.1002/oby.22759
12. McKenzie, A. I., Reidy, P. T., Nelson, D. S., Mulvey, J. L., Yonemura, N. M., Petrocelli, J. J., Drummond, M. J. (2020). Pharmacological inhibition of TLR4 ameliorates muscle and liver ceramide content after disuse in previously physically active mice. Am J Physiol Regul Integr Comp Physiol, 318(3), R503-R511. doi:10.1152/ajpregu.00330.2019
13. Reidy, P. T., Yonemura, N. M., Madsen, J. H., McKenzie, A. I., Mahmassani, Z. S., Rondina, M. T., Drummond, M. J. (2019). An accumulation of muscle macrophages is accompanied by altered insulin sensitivity after reduced activity and recovery. Acta Physiol (Oxf), 226(2), e13251. doi:10.1111/apha.13251

2019

1. Reidy, P. T., McKenzie, A. I., Mahmassani, Z. S., Petrocelli, J. J., Nelson, D. B., Lindsay, C. C., Drummond, M. J. (2019). Aging impairs mouse skeletal muscle macrophage polarization and muscle-specific abundance during recovery from disuse. Am J Physiol Endocrinol Metab, 317(1), E85-E98. doi:10.1152/ajpendo.00422.2018
2. Samson, S. C., Elliott, A., Mueller, B. D., Kim, Y., Carney, K. R., Bergman, J. P., Mendoza, M. C. (2019). p90 ribosomal S6 kinase (RSK) phosphorylates myosin phosphatase and thereby controls edge dynamics during cell migration. J Biol Chem, 294(28), 10846-10862. doi:10.1074/jbc.RA119.007431
3. Yoo, J. H., Brady, S. W., Acosta-Alvarez, L., Rogers, A., Peng, J., Sorensen, L. K., Grossmann, A. H. (2019). The Small GTPase ARF6 Activates PI3K in Melanoma to Induce a Prometastatic State. Cancer Res, 79(11), 2892-2908. doi:10.1158/0008-5472.CAN-18-3026
4. Zhang, P., Holowatyj, A. N., Roy, T., Pronovost, S. M., Marchetti, M., Liu, H., Edgar, B. A. (2019). An SH3PX1Dependent Endocytosis-Autophagy Network Restrains Intestinal Stem Cell Proliferation by Counteracting EGFR-ERK Signaling. Dev Cell, 49(4), 574-589 e575. doi:10.1016/j.devcel.2019.03.029

2018

1. Abdul-Wajid, S., Demarest, B. L., & Yost, H. J. (2018). Loss of embryonic neural crest derived cardiomyocytes causes adult onset hypertrophic cardiomyopathy in zebrafish. Nat Commun, 9(1), 4603. doi:10.1038/s41467018-07054-8
2. Chavez, D. R., Snow, A. K., Smith, J. R., & Stanfield, G. M. (2018). Soma-germ line interactions and a role for muscle in the regulation of C. elegans sperm motility. Development, 145(24). doi:10.1242/dev.167734
3. Klatt Shaw, D., Gunther, D., Jurynec, M. J., Chagovetz, A. A., Ritchie, E., & Grunwald, D. J. (2018). Intracellular Calcium Mobilization Is Required for Sonic Hedgehog Signaling. Dev Cell, 45(4), 512-525 e515. doi:10.1016/j.devcel.2018.04.013
4. Mimche, P. N., Lee, C. M., Mimche, S. M., Thapa, M., Grakoui, A., Henkemeyer, M., & Lamb, T. J. (2018). EphB2 receptor tyrosine kinase promotes hepatic fibrogenesis in mice via activation of hepatic stellate cells. Sci Rep, 8(1), 2532. doi:10.1038/s41598-018-20926-9
5. Reidy, P. T., McKenzie, A. I., Mahmassani, Z., Morrow, V. R., Yonemura, N. M., Hopkins, P. N., Drummond, M. J. (2018). Skeletal muscle ceramides and relationship with insulin sensitivity after 2 weeks of simulated sedentary behaviour and recovery in healthy older adults. J Physiol, 596(21), 5217-5236. doi:10.1113/JP276798
6. Sefton, E. M., Gallardo, M., & Kardon, G. (2018). Developmental origin and morphogenesis of the diaphragm, an essential mammalian muscle. Dev Biol, 440(2), 64-73. doi:10.1016/j.ydbio.2018.04.010
7. Zitnay, J. L., Reese, S. P., Tran, G., Farhang, N., Bowles, R. D., & Weiss, J. A. (2018). Fabrication of dense anisotropic collagen scaffolds using biaxial compression. Acta Biomater, 65, 76-87. doi:10.1016/j.actbio.2017.11.017
8. Zitnay, J. L., & Weiss, J. A. (2018). Load transfer, damage, and failure in ligaments and tendons. J Orthop Res, 36(12), 3093-3104. doi:10.1002/jor.24134

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HSC Imaging Core Newsletters

February 2021

Cell Imaging Core

Email: support.cellimaging@cores.utah.edu

Phone: 801-587-7964