Transgenic & Gene Targeting Mouse Core Facility
The Transgenic & Gene Targeting (TG) Mouse Core Facility provides state of the art services to generate genetically modified mouse research models. The TG Mouse core uses CRISPR technology to generate knockout, knockin, and conditionally targeted alleles in mice. This method allows for the efficient and relatively inexpensive generation of mice with specific genetic mutations. Other services include conventional targeting of mouse ESCs, injection of ESCs to make germline chimeras, and production of traditional transgenic mice using random genomic insertion methods. In addition to producing novel mouse models, the TG Mouse core provides services for common mouse procedures including embryo and sperm cryopreservation, in vitro fertilization (IVF), karyotyping of ESCs, rederivation of mice from frozen embryos and derivation of primary mouse ESCs.
The TG Mouse core staff works closely with University of Utah regulatory groups and is in compliance with strict IACUC and USDA guidelines.
Hours of Operation
8:00 am to 5:00 pm
Monday – Friday
Generation of Genetically Modified Mouse Lines
|Pronuclear Injection||C57BL/6J||$5,500||Implantation of >200 |
|F1||$4,500||Implantation of >200 |
|Embryo Electroporation||F1||$2,250||Implantation of >125|
|C57/Bl6J||$2,000||Implantation of >125|
|Validation of CRIPSR|
reagents (per condtion)
|F1||$220||>50 electroporated embryos|
|C57/Bl6J||$270.00||>50 electroporated embryos|
|CRISPR ESC Targeting||F1||$3,150||192 clones from CRISPR|
|C57/Bl6J||$3,700||192 clones from CRISPR|
|Traditional ESC Targeting||F1||$5,400||192 selected clones|
|C57/Bl6J||$5,950||192 selected clones|
|Blastocyst Injection||F1||$6,000||>2 chimeras|
|Breeding to N1 generation||N/A||$495||Outcrossing of 4 founder mice|
Service fees include the purchase of donor female mice, superovulation, microinjection, microsurgery procedures, ear tissue collection for DNA isolation and animal housing up until weaning. Users will be charged cage per diems for mice that remain in the core post weaning.
These fees do not include CRISPR reagents, transgenic constructs, genotyping, and DNA sequencing. The Mutation Generation and Detection (MGD) Core provides these molecular services or users can also supply their own reagent for injection.
The total cost to generate a novel mouse model from start to the sequenced heterozygous N1 generation is $9,000-$12,000 and depends on the mouse strain and edit type.
|Rederivation of frozen embryos||C57BL/6J||$880||Minimum of 2 carriers|
|F1||$880||Minimum of 2 carriers|
|IVF||F1||$1,440||Minimum of 2 carriers|
|C57BL/6J||$1,650||Minimum of 2 carriers|
|Blastocyst Injection||F1||$6,000||>2 chimeras|
To recover a frozen line please email the core director to coordinate the transfer of the frozen sperm, embryos or ESCs to the core. Users are welcome to ship samples directly to the core.
Core Shipping Address:
University of Utah
Transgenic Gene-Targeting Mouse Facility
15 North 2030 East
Bldg. 533, Room 7470
Salt Lake City, UT 84112
|Sperm Cryopreservation||$625||Live preserved sperm (20 straws) and|
analysis of sperm quality before and after freeze
|Embryo Cryopreservation||$1250||Live frozen embryos (~30-40 embryos) and post freeze viability assessment|
To initiate a sperm cryopreservation service please submit a cryopreservation request form (Resource System Login Required). Upon receiving the completed form, we will submit a request in eSirius to transfer the mice to the core for the procedure. For embryo cryopreservation requests please email the core director: email@example.com
Transgenic Recommended Training Documents
This facility has no recommended training documentation. Please contact the facility directly for information about services and available training
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Citing Our Facility
We would like to thank you for acknowledging the our facility. This recognition allows us to highlight the impact of your work and demonstrates the important contributions of our facility makes to research across the University of Utah. The recognition our core receives from your acknowledgments also aids in receiving grants and further funding for equipment and services we can provide to our users.
Self-Run Services / Instrumentation Usage:
In published papers that used instruments at our facility and notably involved staff members please use the following format:
In published papers where a staff member assisted you in addition to the requested services please use the following format:
For publications resulting from collaborations that assisted with the methodologies, planning process and execution of your experiment in addition to equipment usage we require Co-author attribution on your publication for our facility and any staff members who provided substantial contributions to the originating project.