Metabolic Phenotyping

We are pleased to announce that the Metabolic Phenotyping Core is now offering glucose and insulin clamps for mice and rats, please refer to the website to schedule you runs

 

The Metabolic Phenotyping Core offers several services to help investigators evaluate metabolic phenotypes in multiple model organisms. Our services are offered to the University of Utah research community and off-campus researchers. Services include mitochondrial bioenergetics (Seahorse (XFe96 and XF24)for cells and isolated mitochondria), determination of whole animal energy expenditure using the Columbus Instruments Oxymax Lab Animal Monitoring System, determination of body composition by NMR, determination of circulating metabolite and hormone concentrations using the MAGPIX magnetic based system that can measure up to 50 analytes in a 25µl sample. We are also willing to consult on more complex projects that require more detailed in vivo metabolic phenotyping such as glucose clamps and advanced cardiovascular monitoring on a case-by -case basis. We are also happy to offer protocol consultation as needed.

If you would like to make a reservation to use our equipment or services, please go to resource.cores.utah.edu.

Please acknowledge the University of Utah Metabolic Phenotyping Core when presenting data and obtained using the core services. Thank you for adherence to this policy.
“Work was performed by the Metabolic Phenotyping Core, a part of the Health Sciences Cores at the University of Utah.

Thank you.

Services We Offer

Pricing of Offered Services

Service Application Pricing for U of U users Pricing for non-U of U users Notes
NMR Body Composition $120/hour Academic: $180/hour *Final rate is per hour
Non-Academic: $240/hour
Glucose Tolerance Tests Glucose clearance after an IP injection of glucose. Maximum is 12 mice. $31/mouse Academic: $47/mouse *Final rate is for one mouse
Non-Academic: $62/mouse
GTT + Blood Maximum is 8 mice. $41 Academic: $62 *Final rate is for one mouse
Non-Academic: $82
Insulin Tolerance Tests Glucose clearance after an IP injection of insulin. Maximum is 12 mice. $31/mouse Academic: $47/mouse *Final rate is for one mouse
Non-Academic: $62/mouse
Metabolic Chambers Whole body O2 consumption, CO2 $93/mouse for 72 hours housing Academic: $141/mouse for 72 hours housing *Final rate is for one mouse
Non-Academic: $186/mouse for 72 hours housing
Seahorse XFe96 Analyzer Oxygen consumption rate & extra-cellular acidification rate in isolated mitochondria, cultured cells.

$265/plate for cells.

Self-Run Price: $215

Academic: $401/plate for cells *Final rate is per plate
Non-Academic: $531/plate for cells
Seahorse XF24 Analyzer Oxygen consumption rate & extra-cellular acidification rate in isolated mitochondria, cultured cells, tissue sections and worms $228/plate for cells; $257/plate for tissue; Self-Run Price: $180/plate for cells; $209/plate for tissue Academic: $344/plate for cells;$388/tissue; Non-Academic: $400/plate for cells; $420/plate for tissues *Final rate is for plate
MAGPIX Cytokines, chemokines, hormones, and angiogenesis $350/plate (kit purchased separately) Non-Academic: $700/plate (kit purchased separately) *Final rate for each run minus the kit. The kit will vary for each user.
Luminex 200 Cytokines, chemokines, hormones, angiogenesis, gene expression $425/plate (kit purchased separately) Academic: $642/plate (kit purchased separately) *Final rate for each run minus the kit. The kit will vary with each user.
Non-Academic: $850/plate (kit purchased separately)
Insulin ELISA *Kit is not included $345 Academic: $521 *Final rate is for one 96-well plate
Non-Academic: $1,200
Insulin and Glucose Clamps In vivo Insulin Sensitivity assessment *Typically to compare groups,6-8 animals/group is needed.Prices do not include cost for shipping or quarantine
Hyperinsulinemic euglycemic clamo with [33 – H] glucose $329 Academic: $493.5
Non-Academic: $658
Hyperinsulinemic euglycemic clamo with [23 – H] deoxyglucose $415 Academic: $622.5
Non-Academic: $830
Hyperinsulinemic euglycemic clamo with [33 – H] glucose and [14C]deoxyglucose $431 Academic: $646.5

Non-Academic: $862

Hyperglycemic clamp$447Academic: $670.5 Non-Academic: $894

 

Body Composition

NMR Bruker Minispec

Measures body composition analysis in non-anesthetized mice (no imaging capability). The NMR is located in Emma Eccles Jones Medical Research Building (EEJ, bldg 565). Any mice in EEJ can be used with the NMR. If your mice are outside of this facility there is a 4 week isolation time before they can be transferred to EEJ.

Pricing:

  • U of U Users: $120/hr
  • Non-U of U Users: academic: $180/hr; non-academic: $240/hr

Reserve for Use
How To Use NMR

NMR-BrukerMinispec

Whole Body Metabolism, Temperature, and Movement

Glucose Tolerance Test and Insulin Tolerance Test

 

The most commonly used method for assessing glucose homeostasis in rodent is the glucose tolerance test (GTT). The glucose (1g of glucose/kg) will be given intraperitoneally after 6 hour fasting. Blood samples are taken at intervals (0,5,15,30, 60, 120 minutes post injection) to determine how quickly the glucose is cleared from the blood.

Insulin tolerance test (ITT) is technically very similar to the GTT as it involves monitoring blood glucose levels over time, but in response to insulin administration(0.75U/kg) rather than glucose loading. ITTs are usually carried out in 6 hour fasted animals and blood glucose levels are measured every 5 min for 60-90 min following insulin injection.

Reserve for Use

 

Inirect Colorimetry using CLAMS Metabolic Cages

InirectColorimetry

We have 8 metabolic chambers available that measure x-, y- and z-axis movement; food and water consumption; heat production; O2 consumption; CO2 production in mice. Availability varies so please schedule as far in advance as possible.

The Columbus Instruments (Columbus, OH) mouse metabolic chamber is an open circuit system that directly measures five parameters over a 72 hour period.

  1. Oxygen consumption
  2. Carbon dioxide production
  3. Food intake
  4. Water intake
  5. Activity over X Y & Z axis

Gas measurements are performed on an open system, meaning that air is pumped through the test chambers and gasses measured prior to being expelled into the atmosphere. The percent O2 and CO2 gas levels of the test chamber environment are measured periodically and compared to an external source.

Gas exchange is derived by monitoring both flow and the difference in gas concentration appearing between inlet and outlet of a sealed chamber through which flows a known mass of air. Knowledge of air mass and gas fractions provide the basic information required for the determination of volumes of both O2 and CO2 present at the inlet and outlet. Subtraction of the respective inlet and outlet volumes yield the volume of oxygen consumed and the volume of carbon dioxide produced. The ratios of produced and consumed gasses provides the Respiratory Exchange Ratio (RER) which should fall between 0.7-1.0 depending on the dietary mixture of fats and carbohydrates consumed.

Food intake is measured and recorded using a modified electronic balance (Mettler-Toledo).

Liquid intake is measured using a Volumetric Drinking Monitor (VDM) which operates by dispensing a measured amount of liquid to a sipper tube. Liquid consumed from the sipper tube is electronically monitored, recorded and replaced.

Movement is detected by three sets of light beams (8 beams per set) that measure activity on an X Y & Z axis. A record is made each time the beam path is broken by the animal. Total and ambulatory movements are recorded separately.

Raw data are collated using Microsoft Excel spreadsheet software.

Please be aware that in order for us to accommodate you metabolic cages run, we will appreciate if you can contact us as soon as you set up your experiment so we can help you schedule you run ahead.

Reserve for Use

 

Mitochondrial Bioenergies

Seahorse XF24 Flux Analyzer


The Seahorse Extracellular Flux (XF) Analyzer performs bioenergetic studies in small material samples. XF Analyzers non-invasively profile the metabolic activity of cells or isolated mitochondria, providing data on oxygen consumption, glycolysis rates, ATP turnover and respiratory capacity. More details can be obtained at: http://www.seahorsebio.com/?gclid=CLDfwt7WuKkCFUnr7QodL3pVoA

Pricing:

  • U of U users: $228/plate for cells; $257/plate for tissue; Self-Run Price: $180/plate for cells, $209/plate for tissue
  • Non-U of U users:
    • Academic: $344/plate for cells; $388/plate for tissue
    • Non-Academic: $400/plate for cells; $420/plate for tissue

 

Seahorse XFe96 Analyzer

The Seahorse XFe96 Analyzer provides the highest capacity for XF assays at the lowest per-sample cost. The Seahorse XFe96 enables a large number of experimental groups per assay, making it ideal for labs seeking to maximize design flexibility and sample throughput. The Seahorse XFe96 Analyzer can analyze individual spheroids with the Seahorse XFe96 Spheroid Microplate. More details can be obtained at: http://www.seahorsebio.com/?gclid=CLDfwt7WuKkCFUnr7QodL3pVoA

 

Standard (Core Run) Self Run
Core Provides Cartridge and cell culture plate
Calribrant and XF Assay Media
Mito stress and Glycolysis test kit
Cartridge and cell culture plate
Calribrant
User Provides Sample XF Assay Media
Mito stress or Glycolysis test kit
Sample

Pricing:

  • U of U users: $265/plate for cells; Self-Run: $215
  • Non-U of U users:
    • Academic: $401/plate for cells
    • Non-Academic: $531/plate for cells

Reserve for Use
XF data analysis software (Wave)
Data analysis (Report generator)
Seahorse XF Analyzer Selection Guide
Cell line Density
XF24 Cell Seeding
XFe96 Cell Seeding
Types of Assay

 

Respirometry/Isolated Mitochondria/ATP

Respirometry includes measuring oxygen consumption rates and ATP production in saponin permeabilized tissues from the heart and soleus muscle, and from isolated mitochondria from any tissue.

Pricing:

  • U of U users: $46/isolation & respiration; $92/isolation & respiration + ATP
  • Non-U of U users:
    • Academic: $92/isolation & respiration; $184/isolation & respiration + ATP
    • Non-Academic: $138/isolation & respiration; $276/isolation & respiration + ATP

Reserve for Use

 

 

Respirometry/Isolated Mitochondria/ATP

Respirometry includes measuring oxygen consumption rates and ATP production in saponin permeabilized tissues from the heart and soleus muscle, and from isolated mitochondria from any tissue.

Pricing:

  • U of U users: $46/isolation & respiration; $92/isolation & respiration + ATP
  • Non-U of U users:
    • Academic: $92/isolation & respiration; $184/isolation & respiration + ATP
    • Non-Academic: $138/isolation & respiration; $276/isolation & respiration + ATP

Reserve for Use

 

Biochemistries

MAGPIX

MAGPIX

 

The MAGPIX is a multiplexing machine that uses magnetic bead mapping technology to get concentrations of cytokines in samples (blood, media, and tissue extraction). This multiplexing unit performs up to 50 different tests in a single reaction volume and reads a 96-well-plate in just 60 minutes. This system features an innovative design based on CCD imaging technology that allow for a more compact, robust system. And MAGPIX is supported by a broad menu of commercially available assay kits. As well as reagents to build your own assays.

Advantages

  • Low cost than ELISA
  • Powerful – More than 125 magnetic bead-based assay kits
  • Multiplex – Measure up to 50 target in 25ul of samples

 

To know more detail, please contact the core with a kit or a list of cytokines you want to test.

Pricing:

  • U of U users: $350/plate (kit purchased separately)
  • Non-U of U users:
    • Academic: $525/plate
    • Non-Academic: $700/plate (kit purchased separately)

Reserve for Use
Extraction Protocol

 

Luminex 200

luminex-100-200

“The Luminex 100/200 System is a flexible analyzer based on the principles of flow cytometry. The system enables you to multiplex (simultaneously measure) up to 100 analytes in a single microplate well, using very small sample volumes. The system delivers fast and cost-effective bioassay results on many assay formats including nucleic acid assays, receptor-ligand assays, immunoassays and enzymatic assays.

“The Luminex 100/200 System is the combination of three core xMAP Technologies. The first is xMAP microspheres, a family of 100 fluorescently dyed 5.6 micron-sized polystyrene microspheres that act as both the identifier and the solid surface to build the assay. The second is a flow cytometry-based instrument, the Luminex 100/200 analyzer, which integrates key xMAP detection components such as lasers, optics, fluidics and high-speed digital signal processors. The third component is the xPONENT® software, which is designed for protocol-based data acquisition with robust data regression analysis.” (http://www.luminexcorp.com/Products/Instruments/Luminex100200/)

 

Pricing:

  • U of U users: $425/plate (kit purchased separately)
  • Non-U of U users:
    • Academic: $642/plate (kit purchased separately)
    • Non-Academic: $850/plate (kit purchased separately)

Reserve for Use

Hours of Operation

Monday-Friday 8:30am-5pm

Location

Hours of Operation

Monday-Friday from 8am-5pm

Location

15N 2030E Radiology building 585 room 151

Salt Lake City, UT 84112

Staff

Anil Laxman Ph.D, Director
801-585-0860
alaxman@cores.utah.edu