Cell Imaging

The Cell Imaging Facility provides training and consultation on the use of confocal microscopy, widefield automated microscopy, and software analysis tools for quantitative analysis of image data. The facility has four locations (HCI, HSC, Biology, Biochemistry) each with confocal and widefield capabilities. There are several options for confocal microscopy of fixed or live samples, including Nikon A1, A1R, Leica SP8 white light, Olympus FV1000s, Bruker Ultima 2 Photon and a Zeiss 880 Airyscan. There are two Nikon Ti automated microscopes with laser autofocus and stage incubators in addition to two Olympus widefield CCD systems. These instruments run using a variety of proprietary software platforms and some multifunctional image capture programs such as Metamorph, Micromanager and Imaris software are available for 2D and 3D analysis of image data.

Web calendar for scheduling appointments: This system is core wide and uses the campus UNID system. Upon first login you will be asked for information and for which PI there is an authorized chartfield registered with the cores office. If you have problems logging in contact billing at billing@cores.utah.edu

Before working with us, please take the time to read our Microscopy Core Policies. To make a reservation to use our equipment or services, please go to resource.cores.utah.edu. Please arrange for a training appointment with core staff to become approved to use the instruments. Instruments are available 24/7 to appropriately trained users.

Acknowledgments to the Cell Imaging Core

We would like to thank you for acknowledging the University of Utah Cell Imaging Core. This recognition allows us to highlight the impact of your work and demonstrates the important contributions the Cell Imaging Core makes to research across the University of Utah. The recognition our core receives from your acknowledgments also aids in receiving grants and further funding for equipment we can provide to our users.

Self-Users: please mention in your published papers the use of instruments via the University of Utah Cell Imaging Core and any notable staff member.

We acknowledge Cell Imaging Core at the University of Utah for use of equipment (insert microscope(s) or instrument details here), and thank (insert any notable staff member – if desired) for their assistance.

Assisted Imaging: Please mention in your published papers the associate of our staff who took your images for you.

We acknowledge Cell Imaging Core at the University of Utah for use of equipment (insert microscope(s) or instrument detail here), and thank (insert staff member-required) for their assistance in image acquisition.

Collaboration: Co-author acknowledgment, collaborations that assist with the methodologies and planning process of your experiment as well as instrument use. At this depth of assistance, this type of recognition is required.

A variety of instrumentation is available at the Cell Imaging Core.  Click on links below for further information on these systems.

HSC Building 585/586
Confocal Microscopes

Nikon A1

Room 59
U of U Personnel Rates: $25/hr; $12.50 nights & weekends

More Info

Schedule for Use
niona1

Nikon A1R

Room 54
U of U Personnel Rates: $25/hr; $12.50 nights & weekends

More Info

Schedule for Use
image4

Prairie Multi Confocal

Room 48B
U of U Personnel Rates: $25/hr; $12.50 nights & weekends

More Info

Schedule for Use
newphoton

Prairie Ultima 2 Photon Confocal

Room 48D
U of U Personnel Rates: $25/hr; $12.50 nights & weekends

More Info

Schedule for Use
2ph
Widefield Microscopes

Nikon Widefield Microscope

Location: Room 56
U of U Personnel Rates: $12.5/hr

More Info

Schedule for Use
widefield

EVOS FL

Location: Room 54
U of U Personnel Rates: $12.5/hr

More Info

Schedule for Use
image5a
Slide Scanning

Axio Scan.Z1

Location: Room 60
U of U Personnel Rates: $25/hr; $12.50/hr nights & weekends

 

Schedule for Use
HCI Core Location

A variety of instrumentation is available at the HCI location. These include a Leica SP8 white light laser confocal and automated widefield.  Click on links below for further information on these systems. Mike Bridge and Chris Rodesch (801-633-6589) have office hours there two days a week.

Leica SP8 White light Laser Confocal:

Location: Room 1480
U of U Personnel Rates:$25/hr; $12.5 nights & weekends

More Info

Schedule for Use

Leica SP8 405-488-561-633 Laser Confocal:

Location: Room 1440
U of U Personnel Rates:$25/hr; $12.5 nights & weekends

More Info

Schedule for Use

Nikon Widefield Microscope

Location: Rm 1430
U of U Personnel Rates:$12.5/hr

More Info

Schedule for Use

New Workstation

Location: Rm 1470
U of U Personnel Rates:$12.5/hr

More Info

Schedule for Use

Alice B. Skaggs Biology Building

Vutara

Location: Rm
U of U Personnel Rates:$12.5/hr

More Info

Schedule for Use

Crocker Science Center

A variety of instrumentation is available at the CSC location at Room 032.  Click on links below for further information on these systems

Zeiss 880 Airy Scan:

Location: Room 032
U of U Personnel Rates:$25/hr; $12.5 nights & weekends

More Info

Schedule for Use

COMING SOON – The Zeiss Elyra:

Location: Room 032
U of U Personnel Rates:$25/hr; $12.5 nights & weekends

 

Schedule for Use

James Sorenson Molecular Biotechnology Building

Olympus FV1000

Location: Currently located in SMBB
U of U Personnel Rates: $25/hr; $12.50 nights & weekends

More Info

Schedule for Use

ASB – Aline Wilmot Skaggs Biology Building: map

Number: 82
Code: ASB
Address: 259 S 1400 E Salt Lake City, UT 84112

 

CSC – Crocker Science Center: map

Number: 5
Code: CSC
Address: 1390 Presidents Cir Salt Lake City, UT 84112

 

EEJMRB – E.E. Jones Medical Research: map

Number: 565
Code: EEJMRB
Address: 15 N Medical Dr East Salt Lake City, UT 84112

 

HCI – Huntsman Cancer Institute: map

Number: 555
Code: HCI
Address: 2000 Circle of Hope Salt Lake City, UT 84103

 

HSC – Health Science Center: map

Number: 585
Code: RB LAB
Address: 30 N 2030 E Salt Lake City, UT 84112

 

SMBB – Sorenson Molecular Biotechnology (USTAR): map

Number: 151
Code: SMBB
Address: 36 S Wasatch Dr Salt Lake City, UT 84112

To Set Up a User Account:

Account Setup

Booking Calendar:

Booking Calendar
  1. Fill out and submit a Work Authorization form.  Work Authorization Form.
    • If you need help navigating the form, a ‘How to’ guide is available under the drop down menu labeled ‘Work Auth Forms
    • For additional questions please contact the administrative office at billing@cores.utah.edu
  2. After your authorization is approved, contact us at our email support.cellimaging@cores.utah.edu
  3. Training and an initial project consultation is required and helps us ensure you receive the best images possible that your samples produce.
    • Through our email contact we an give you suggestions on which of our staff bests fits your experiment and goals for training and assistance
    • additional training information is located under the ‘Training Tab’.
  4. Booking on the calendar website
    • Login using your unid (ex. uxxxxxx) and cis password.
    • After logging in, the Cell Imaging Core calendar is located under ‘Resources’ and ‘Cell Imaging’
    • Each Staff member and microscope has a calendar that shows availability that you may book time for your use. Our HSC, HCI and Crocker locations are staffed from 9am-1pm Monday through Friday. Reservations for training and consulations at a specific location (e.g. Morning HCI consolation/training will be with whomever is staffed at that location). If you want an appointment with a specific staff member, choose appointments in the afternoon and the location just like before. For any questions regarding our new calendar system please email us at support.cellimaging@cores.utah.edu.
    • After booking staff member’s time, check the drop down option next to ‘Process’ to add location. Under ‘Data Fields’ and ‘Label add a description of intended use and experiment type.
  5. Microscope
    • First Step: Book time slot with suggested staff for a one-hour mandatory start up project consultation. There you will review goals and plan.
    • Second Step: Book time with both the staff member and suggested microscope simultaneously for initial hardware and software training.
    • Third step: *Optional* After initial training it is suggested to book time with staff for first imaging session.
  6. Building access granted after full training is complete
    • HCI
      • Computer and building access forms will be provided
      • Submit forms per our instructions
    • HSC and CSC
      • You must then go in person with your UID card to our Administrative Office to gain card access to the building and rooms to which you have been granted permissions. To get to our Administrative Office (SOM 5C124), you must go to elevator C in the School of Medicine, go to floor 5, turn immediately right around the corner, and the office is down the hall on the left side.
    • SMBB
      • We will send your information to the SMBB building office.

 

If you have any more questions concerning this process please contact us at support.cellimaging@cores.utah.edu.

Our HSC, HCI, and Crocker locations will be staffed from 9am – 1pm Monday through Friday. Reservations for training and consultations at a location (e.g. Morning HCI Consultation/Training) will be with whomever is staffing the location you reserve. If you want an appointment with a specific staff member choose appointments in the afternoon and the location just like before.

 

HSC – Health Science Center: http://bit.ly/2Oakzdi

Number: 585

Address: 30 N 2030 E Salt Lake City, UT 84112

Office Phone Number:801-587-7964

Office Room numbers:Room 55 – Michael Bridge

Room 48a – Isabelle Harward

Room 48e – Christopher Rodesch

Room 158 (second Floor) – Xiang Wang

All offices, except Xiang’s, are located on the bottom floor near microscopy equipment

Email:support.cellimaging@cores.utah.edu

 

HSC is our main facility for the Cell Imaging Core, it is  manned in the morning by Michael Bridge 9am-1pm for consultations, and he can be scheduled at any other location after 1pm.

 

 

 

HCI – Huntsman Cancer Institute: http://bit.ly/2MC4koV

Number: 555
Address: 2000 Circle of Hope Salt Lake City, UT 84103

Office Phone Number: 801-585-0106

Office Room Number:1460

Xiang’s office is located in Research North HCI, first floor, near all microscopy equipment

Emailsupport.cellimaging@cores.utah.edu

Our HCI satellite facility is manned in the morning by Xiang Wang 9am-1pm for consultations, and he can be scheduled at any other location after 1pm.

 

 

 

CSC – Crocker Science Center: http://bit.ly/2tD9N7r

Number: 5
Address: 1390 Presidents Cir Salt Lake City, UT 84112

Office Phone Number: 801-587-0313

Office Room Number:030

Christopher’s office is located on the lower level (floor LL), through the double doors, and is next to the doors leading to the microscopy equipment.

Email:support.cellimaging@cores.utah.edu

 

Our CSC satellite facility is manned in the morning by Chris Rodesch 9am-1pm for consultations, and he can be scheduled at any other location after 1pm.

Publications

2018

  1. Abdul-Wajid, S., Demarest, B. L., & Yost, H. J. (2018). Loss of embryonic neural crest derived cardiomyocytes causes adult onset hypertrophic cardiomyopathy in zebrafish. Nat Commun, 9(1), 4603. doi:10.1038/s41467018-07054-8
  2. Chavez, D. R., Snow, A. K., Smith, J. R., & Stanfield, G. M. (2018). Soma-germ line interactions and a role for muscle in the regulation of C. elegans sperm motility. Development, 145(24). doi:10.1242/dev.167734
  3. Klatt Shaw, D., Gunther, D., Jurynec, M. J., Chagovetz, A. A., Ritchie, E., & Grunwald, D. J. (2018). Intracellular Calcium Mobilization Is Required for Sonic Hedgehog Signaling. Dev Cell, 45(4), 512-525 e515. doi:10.1016/j.devcel.2018.04.013
  4. Mimche, P. N., Lee, C. M., Mimche, S. M., Thapa, M., Grakoui, A., Henkemeyer, M., & Lamb, T. J. (2018). EphB2 receptor tyrosine kinase promotes hepatic fibrogenesis in mice via activation of hepatic stellate cells. Sci Rep, 8(1), 2532. doi:10.1038/s41598-018-20926-9
  5. Reidy, P. T., McKenzie, A. I., Mahmassani, Z., Morrow, V. R., Yonemura, N. M., Hopkins, P. N., Drummond, M. J. (2018). Skeletal muscle ceramides and relationship with insulin sensitivity after 2 weeks of simulated sedentary behaviour and recovery in healthy older adults. J Physiol, 596(21), 5217-5236. doi:10.1113/JP276798
  6. Reidy, P. T., McKenzie, A. I., Mahmassani, Z. S., Petrocelli, J. J., Nelson, D. B., Lindsay, C. C., Drummond, M. J. (2019). Aging impairs mouse skeletal muscle macrophage polarization and muscle-specific abundance during recovery from disuse. Am J Physiol Endocrinol Metab, 317(1), E85-E98. doi:10.1152/ajpendo.00422.2018
  7. Samson, S. C., Elliott, A., Mueller, B. D., Kim, Y., Carney, K. R., Bergman, J. P., Mendoza, M. C. (2019). p90 ribosomal S6 kinase (RSK) phosphorylates myosin phosphatase and thereby controls edge dynamics during cell migration. J Biol Chem, 294(28), 10846-10862. doi:10.1074/jbc.RA119.007431
  8. Sefton, E. M., Gallardo, M., & Kardon, G. (2018). Developmental origin and morphogenesis of the diaphragm, an essential mammalian muscle. Dev Biol, 440(2), 64-73. doi:10.1016/j.ydbio.2018.04.010
  9. Yoo, J. H., Brady, S. W., Acosta-Alvarez, L., Rogers, A., Peng, J., Sorensen, L. K., Grossmann, A. H. (2019). The Small GTPase ARF6 Activates PI3K in Melanoma to Induce a Prometastatic State. Cancer Res, 79(11), 2892-2908. doi:10.1158/0008-5472.CAN-18-3026
  10. Zhang, P., Holowatyj, A. N., Roy, T., Pronovost, S. M., Marchetti, M., Liu, H., Edgar, B. A. (2019). An SH3PX1Dependent Endocytosis-Autophagy Network Restrains Intestinal Stem Cell Proliferation by Counteracting EGFR-ERK Signaling. Dev Cell, 49(4), 574-589 e575. doi:10.1016/j.devcel.2019.03.029
  11. Zitnay, J. L., Reese, S. P., Tran, G., Farhang, N., Bowles, R. D., & Weiss, J. A. (2018). Fabrication of dense anisotropic collagen scaffolds using biaxial compression. Acta Biomater, 65, 76-87. doi:10.1016/j.actbio.2017.11.017
  12. Zitnay, J. L., & Weiss, J. A. (2018). Load transfer, damage, and failure in ligaments and tendons. J Orthop Res, 36(12), 3093-3104. doi:10.1002/jor.24134
Hours of Operation

Monday-Friday from 9am-5pm

Location

Radiobiology Building (Bldg 585), Room 48
30 N 2030 East
Salt Lake City, UT 84112

Staff
xiang-cell

Xiang Wang Ph.D., Co-Director
801-587-7964
support.cellimaging@cores.utah.edu

xiang.wang@cores.utah.edu
ChrisR-2448x2028crop

Chris Rodesch Ph.D., Co-Director
801-587-7964
support.cellimaging@cores.utah.edu

crodesch@cores.utah.edu
mike
Michael J. Bridge Ph.D., Research Associate
801-587-7964
support.cellimaging@cores.utah.edu

mike.bridge@utah.edu

 

isabelle-cell
Isabelle Harward, Microscope Technician
801-587-7964
support.cellimaging@cores.utah.edu

isabelle.harward@utah.edu
Oversight Committee

Chair:
Bruce Edgar, Professor, Oncological Sciences

Committee:

James Cox, Associate HSC Cores Director
Alex Shcheglovitov, Assistant Professor, Neurobiology and Anatomy
Mark Smith, Research Assistant Professor, Oncological Sciences
Markus Babst, Professor, Biological Sciences
Michelle Mendoza, Associate Professor, Oncological Sciences
Minna Roh-Johnson, Assistant Professor, Dept. of Biochemistry
Sophie Caron, Assistant Professor, Biological Sciences
Gabrielle Kardon, Professor, Human Genetics

Please send feedback about future plans, core policies issues with equipment and usage to the committee if you think it is something we should consider at our meetings.