Utah Proteomics Affinity Group
presents the
1st Annual Utah Proteomics
Summer Short Course
July 14-17, 2025
Our Fundamentals in Proteomics course will familiarize participants with the foundations of mass spectrometry (MS)-based proteomics experimental design, sample preparation, LC-MS/MS equipment handling, and data analysis tools. The course will include a balanced mix of classroom lectures from University of Utah and Brigham Young University Investigators U of U and BYU Investigators and hands-on training!
Course Location:
Crocker Science Center
University of Utah
1390 Presidents’ Circle
Salt Lake City, UT 84112
Course Description:
- Introduction to MS-based proteomics, experimental design, and common assays
- Hands-on protein digestion techniques for bottom-up proteomics.
- Theory and applications of nano-electrospray liquid chromatography (LC)-MS on the ThermoFisher Scientific Exploris 480 and the Bruker timsTOF Pro2.
- Fundamentals of quantitative proteomics using both isobaric labeling and label free data independent analysis.
- Hands-on data analysis techniques using the open-source software packages Fragpipe 22.0, DIA-NN 1.9.1, and Perseus 2.0.11.
Registration fee $100, all materials and lunches included! This course is limited to 20 participants. Prospective participants will be waitlisted after capacity has been reached.
We would like to thank our sponsors , the U of U CoP, HCI, CVRTI, and HSC Cores, for their generous support of the proteomics research community in the state of Utah.
Organizers
| Day 1 | |
|---|---|
| 8am – 8:45am: | Intro to Proteomics (lecture) – Dr. Brandon Gassaway (BYU) |
| 8:45am – 9am: | Break |
| 9am – 10am: | Proteomics Sample Preparation Strategies (lecture) – Dr. Martin Golkowski (U of U) |
| 10:30am – 1pm: | Protein Digestion (wet lab) – Dr. Allison Manuel, Dr. Martin Golkowski and Mr. Ryan Bia (U of U) |
| 1 – 2pm: | Lunch |
| 2pm – 4pm: | Protein Digestion (wet lab) – Dr. Allison Manuel, Dr. Martin Golkowski and Mr. Ryan Bia (U of U) |
| Day 2 | |
|---|---|
| 8am – 10:30am: | Finish protein digestion (wet lab) – Dr. Allison Manuel, Dr. Martin Golkowski and Mr. Ryan Bia (U of U) |
| 10:30am – 11:30am: | Intro to LC-MS/MS analysis (lecture) – Dr. Chris Tracy (BYU) |
| 11:30am – 12:30pm: | Proteomics Technologies (lecture) – Dr. Allison Manuel (U of U) |
| 12:30pm – 1:30pm: | Lunch |
| 2pm – 4pm: | LC-MS- Exploris480 and timsTOF (wet lab) – Dr. Allison Manuel and Dr. Martin Golkowski (U of U) |
| Day 3 | |
|---|---|
| 8am – 11am: | Attendee Research Presentations How can proteomics be applied to your current research? |
| 11am – 12pm: | Proteomics Data Analysis (lecture) – Dr. Paul Stewart (U of U) |
| 12pm – 1pm: | Lunch |
| 1pm – 4pm: | DDA Data Analysis (computer lab) – Dr. Paul Stewart (U of U) |
| Day 4 | |
|---|---|
| 8am – 9am: | Proteomics Applications in Pathology Research (lecture) – Dr. Sarah Franklin (U of U) |
| 9am – 10am: | Protein Quantitation using DIA and Isobaric Labeling (lecture) – Dr. Brandon Gassaway (BYU) and Dr. Allison Manuel |
| 10am – 10:30am: | Break |
| 10:30am – 12pm: | DIA Data Analysis (computer lab) – Dr. Martin Golkowski (U of U) |
| 12pm – 1pm: | Lunch |
| 1pm – 4pm: | More Data Analysis! – Dr. Paul Stewart (U of U) |
| Course Evaluation |
Photos will be taken throughout the workshop and posted on our website. Registering for the event implies consent.