Production of mice using injection of CRISPR’s with or without accompanying donor DNA.
Knock out or small donor (<200bp) insertion

Price: $3500 for F1 (CBAxC57Bl6) embryos

Price: $4500 for C57Bl6 embryos

Price: $3500 for any other specific strain (researcher must provide mice)

Large single stranded donor or IoxP insertions

Price: $4000 for F1 (CBAxC57Bl6) embryos

Price: $5000 for C57Bl6 embryos

Price: $4000 for any other specific strain (researcher must provide mice)

 

 

Guaranteed at least 200 implanted, injected eggs

Services provided by the facility:

The transgenic mouse facility will superovulate donor mice, harvest single cell embryos and inject CRISPR reagents provided by the investigators. The Core provides Cas9 protein or will use Cas9 expressing mice. The University of Utah Mutation Generation and Detection Core provides excellent service for design and production of CRISPR guides and donors. Surviving embryos will be implanted into pseudopregnant females.

At least 200 embryos will be injected and implanted, which usually results in approximately 50 pups. Ear biopsies from all pups will be given to the investigator for genotyping. There is no guarantee that resulting pups will be targeted.

Responsibilities of the investigator

  • Discuss experimental design with facility director before initiation of the project.
  • Provide guide RNA’s and donor to the facility. We require at least 1ug of purified donor DNA at a concentration of at least 100ng/ul. DNA must have a 260/280 ratio of 1.8 to 2, and a 260/230 ratio of greater than 2
  • Obtain IACUC approval and mouse space for your project.
  • Submit work authorization form.

CRISPR Production of Mice fee includes:

$3500-$5000 will be charged when pups are born after injection of DNA into embryos.

If there are no correctly targeted pups we will reinject your reagents and charge half.

Any animals left with the facility longer than 3 weeks after ear biopsies have been taken for genotyping will be charged to the investigator at $2.00/ day/ cage of animals.