Procedure |
Fee |
Guarantee |
Time Frame |
Gene Targeting of ES Cells (R1 or G4 ES cells) |
$5000 |
DNA from 192 clones; if no targets we will reEP,
pick 384 clones, charge half |
4 weeks from vector DNA to ES DNA |
Gene Targeting of ES cells (B16 ES cells) |
$5500 |
DNA from 192 clones; if no targets we will reEP,
pick 384 clones, charge half |
4 weeks from vector DNA to ES DNA |
Blastocyst Injection (R1 or G4 ES cells) |
$5500 |
2 or more >80% male chimeras or
germline transmission |
6 weeks from ES cells to chimeras;
3 months to check for germline transmission |
Blastocyst Injection (BI6 ES cells) |
$6000 |
2 or more >50% male chimeras or
germline transmission |
6 weeks from ES cells to chimeras;
3 months to check for germline transmission |
Transgenic pronuclear injection (F1 embryos) |
$3500 |
Implantation of >200 injected eggs |
5 weeks from vector DNA to
earclips for analysis |
Transgenic pronuclear injection (C57BI6 embryos) |
$4500 |
Implantation of >200 injected eggs |
5 weeks from vector DNA to
earclips for analysis |
CRISPR injections no donor/small donor <200bp (F1 embryos) |
$3500 |
Implantation of >200 injected eggs |
5 weeks from CRISPR reagents to
earclips for analysis |
CRISPR injections no donor/small donor <200bp (C57BI6 embryos) |
$4500 |
Implantation of >200 injected eggs |
5 weeks from CRISPR reagents to
earclips for analysis |
CRISPR injections large donor/IoxP’s (F1 embryos) |
$4000 |
Implantation of >200 injected eggs |
5 weeks from CRISPR reagents to
earclips for analysis |
CRISPR injections large donor/IoxP’s donor (C57BI6 embryos) |
$5000 |
Implantation of >200 injected eggs |
5 weeks from CRISPR reagents to
earclips for analysis |
Validation of guide RNA’s (F1 embryos) |
$500 |
Injection or electroporation of up to 3 guides in ~ 100 embryos |
1 week from reciving gRNA’s to lysed blastocysts to analyze |
Validation of guide RNA’s (C57BI6 embryos) |
$750 |
Injection or electroporation of up to 3 guides in ~ 100 embryos |
1 week from receiving gRNA’s to lysed blastocysts to analyze |
ES Cell Line Derivation |
$2000 |
4 ES cell lines |
3 weeks from blastocysts collection to
DNA from ES cell lines for analysis |
Sperm Cryopreservation |
$550 |
Viable sperm |
Immediate |
IVF (F1 eggs) |
$1000 |
Viable pups |
5 weeks from sperm to
earclips for analysis |
IVF (C57BI6 eggs) |
$1500 |
Viable pups |
5 weeks from sperm to
earclips for analysis |
ICSI (intracytoplasmic sperm injection) |
$2500 |
Viable pups |
5 weeks from sperm to
earclips for analysis |
Rederivation of Mouse Lines (embryo transfer) |
$800 |
Live mice |
5 weeks from embryos to
earclips for analysis |
Karyotyping of ES cells |
$250 |
50 spreads counted |
1 week from ES cells to counts |
Exportation of blastocysts |
$150 |
Viable gametes |
1 week |
Cryopreservation of Mouse Embryos |
$1500 |
Viable mouse embryos |
Immediate |
Sperm storage |
$15/cane/year |
|
Billed annually |
Mouse housing/breeding |
$1.25/cage/day |
For mice kept after 4 weeks of age; includes earclipping if necessary |
|
Reclipping for 2nd genotyping |
$1/mouse |
|
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Other projects |
per project basis |
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