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  • HSC Cores
      • Cores Administration
      • Amaxa NucleofectorResource Login
      • Cell ImagingTraining and consultation on use of Imaging Resources
      • DSS – Data Science Servicesprovides data science expertise to the Health Sciences research community
      • DNA/Peptide SynthesisA wide range of routine and specialty oligonucleotides as well as chemically synthesized peptides
      • DNA SequencingProvide DNA Sequencing services
      • Drug Discoveryprovides compound collections for screening, equipment and support
      • Electron Microscopyelectron microscopy (TEM) and scanning electron microscopy (SEM)
      • Flow Cytometry
      • Genomicscost effective genotyping services
      • Human ORFfull-length human open reading frames (ORFs) from the mammalian Genome Collection
      • Iron & Hemeprovides analysis of metals, precursor porphyrins and heme
      • Machine Shoppremiere milling and machining facility
      • Mass Spectrometry & Proteomicsprovide the highest quality mass spectrometry analyses
      • Metabolic Phenotypingoffers several services to help investigators evaluate metabolic phenotypes
      • Metabolomicsprovides metabolite profiling analysis
      • Mutation Generation & Detectionspecializes in providing custom TALEN and Crispr-Cas9 DNA nucleases to induce targeted mutations
      • Nuclear Magnetic ResonanceNMR for data processing, data analysis and structure calculation
      • Preclinical Imagingthe benefits of modern diagnostic medical imaging systems to the studies of anatomy and physiology in small animals
      • Small Animal Ultrasoundthe capability to extend ultrasound imaging to mouse, rat, and other animal models with excellent spatial and temporal resolution
      • Transgenic Gene-Targeting Mouse Facilityto make transgenic mice using pronuclear injection of DNA constructs including BAC DNA into embryos
      • Utah Center for Genetic Discovery
      • 3I-UCGD Core
    • HCI Cores
      • Bioinformatic AnalysisThe Bioinformatic Analysis group assists with the design and analysis of next-generation sequencing and microarray experiments.
      • Biorepository and Molecular Pathologyseamlessly integrating biorepository, histology, and state-of-the-art molecular diagnostic services
      • Cancer Biostatisticsprovides state-of-the-art biostatistics and research design support to laboratory, translational, clinical, and population sciences
      • Genetic Counselingto facilitate clinical, behavioral, and basic science research involving cancer genetics and inherited susceptibility
      • High Throughput Genomicsto support the genomic experimentation and bioinformatic analysis needs
      • Immuno-Oncology NetworkA centralized resource for cutting edge immunological based services for preclinical and clinical studies within Huntsman Cancer Institute, the University of Utah, and beyond.
      • Lab Support Servicesprovides a myriad of research laboratory supplies
      • Preclinical Research Resourcecancer translational research-centered mission
      • Quantitative Cancer ImagingThe CQCI focuses on state-of-the-art clinical and preclinical PET imaging.
      • Research Informaticsresearch informatics shared resources
      • Utah Population Databasein-depth information that supports research on genetics, epidermiology, demography, and public health
    • University Cores
      • Biomedical Informatics Core (BMIC)provides coordination and direction for the information technology resources
      • Biostatistics Centera mission to advance high quality research
      • Clinical Research Compliance and Educationfacilitate processes, provide education, and engage in oversight activities
      • Materials Characterizationaccess to a wide range of analytical instrumentation and services
      • Nanofab – CleanroomProviding equipment, training, and services in nano-fabrication, other research materials, and microelectronics for researchers and industrial customers.
      • Nanofab – Electron Microscopy and Surface Analysis LabProviding access, training and services in electron microscopy (SEM and TEM), materials characterization and surface analysis.
      • NMRCNuclear magnetic resonance (NMR) is a technique that detects the interaction of radio-frequency radiation with a sample
      • Optical Spectroscopyoffers a wide range of analytical instrumentation
      • Research Information Services (RiSe)resource to help faculty, staff, and students in their research pursuits
      • Sirferstable isotope ratio facility for environmental research
      • Utah MR Research Center (UMRC)
    • Service/Recharge Centers
      • ADD – Anticonvulsant Drug Discoveryan established laboratory experienced in the preclinical identification and evaluation of investigational compounds for the treatment of epilepsy
      • (BIDAC) Biomedical Imaging and Data Science Coreproviding advanced image analysis, data analytics and visualization services
      • Cellular Translational Research Core (CTRC)providing stem cell and molecular biology services to investigators
      • Center for Human ToxicologyProvides bioanalytical services to investigators
      • Clinical Services Core (CSC)provides assistance to investigators in the design, planning, budgeting, and performance of human subject research
      • Community Engagement Services Coresupport researchers and leaders in becoming full partners in research projects
      • Genetic Science Learning Center (GSLC)major resources on learning about genetics and other scientific related fields
      • Behavior Health Innovation and Dissemination Centerhigh-quality evidence-based treatment to all
      • Scalable Analytics & Informatics (USAI)provides support to research and operations groups
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    Search:
    • HSC Cores
        • Cores Administration
        • Amaxa NucleofectorResource Login
        • Cell ImagingTraining and consultation on use of Imaging Resources
        • DSS – Data Science Servicesprovides data science expertise to the Health Sciences research community
        • DNA/Peptide SynthesisA wide range of routine and specialty oligonucleotides as well as chemically synthesized peptides
        • DNA SequencingProvide DNA Sequencing services
        • Drug Discoveryprovides compound collections for screening, equipment and support
        • Electron Microscopyelectron microscopy (TEM) and scanning electron microscopy (SEM)
        • Flow Cytometry
        • Genomicscost effective genotyping services
        • Human ORFfull-length human open reading frames (ORFs) from the mammalian Genome Collection
        • Iron & Hemeprovides analysis of metals, precursor porphyrins and heme
        • Machine Shoppremiere milling and machining facility
        • Mass Spectrometry & Proteomicsprovide the highest quality mass spectrometry analyses
        • Metabolic Phenotypingoffers several services to help investigators evaluate metabolic phenotypes
        • Metabolomicsprovides metabolite profiling analysis
        • Mutation Generation & Detectionspecializes in providing custom TALEN and Crispr-Cas9 DNA nucleases to induce targeted mutations
        • Nuclear Magnetic ResonanceNMR for data processing, data analysis and structure calculation
        • Preclinical Imagingthe benefits of modern diagnostic medical imaging systems to the studies of anatomy and physiology in small animals
        • Small Animal Ultrasoundthe capability to extend ultrasound imaging to mouse, rat, and other animal models with excellent spatial and temporal resolution
        • Transgenic Gene-Targeting Mouse Facilityto make transgenic mice using pronuclear injection of DNA constructs including BAC DNA into embryos
        • Utah Center for Genetic Discovery
        • 3I-UCGD Core
      • HCI Cores
        • Bioinformatic AnalysisThe Bioinformatic Analysis group assists with the design and analysis of next-generation sequencing and microarray experiments.
        • Biorepository and Molecular Pathologyseamlessly integrating biorepository, histology, and state-of-the-art molecular diagnostic services
        • Cancer Biostatisticsprovides state-of-the-art biostatistics and research design support to laboratory, translational, clinical, and population sciences
        • Genetic Counselingto facilitate clinical, behavioral, and basic science research involving cancer genetics and inherited susceptibility
        • High Throughput Genomicsto support the genomic experimentation and bioinformatic analysis needs
        • Immuno-Oncology NetworkA centralized resource for cutting edge immunological based services for preclinical and clinical studies within Huntsman Cancer Institute, the University of Utah, and beyond.
        • Lab Support Servicesprovides a myriad of research laboratory supplies
        • Preclinical Research Resourcecancer translational research-centered mission
        • Quantitative Cancer ImagingThe CQCI focuses on state-of-the-art clinical and preclinical PET imaging.
        • Research Informaticsresearch informatics shared resources
        • Utah Population Databasein-depth information that supports research on genetics, epidermiology, demography, and public health
      • University Cores
        • Biomedical Informatics Core (BMIC)provides coordination and direction for the information technology resources
        • Biostatistics Centera mission to advance high quality research
        • Clinical Research Compliance and Educationfacilitate processes, provide education, and engage in oversight activities
        • Materials Characterizationaccess to a wide range of analytical instrumentation and services
        • Nanofab – CleanroomProviding equipment, training, and services in nano-fabrication, other research materials, and microelectronics for researchers and industrial customers.
        • Nanofab – Electron Microscopy and Surface Analysis LabProviding access, training and services in electron microscopy (SEM and TEM), materials characterization and surface analysis.
        • NMRCNuclear magnetic resonance (NMR) is a technique that detects the interaction of radio-frequency radiation with a sample
        • Optical Spectroscopyoffers a wide range of analytical instrumentation
        • Research Information Services (RiSe)resource to help faculty, staff, and students in their research pursuits
        • Sirferstable isotope ratio facility for environmental research
        • Utah MR Research Center (UMRC)
      • Service/Recharge Centers
        • ADD – Anticonvulsant Drug Discoveryan established laboratory experienced in the preclinical identification and evaluation of investigational compounds for the treatment of epilepsy
        • (BIDAC) Biomedical Imaging and Data Science Coreproviding advanced image analysis, data analytics and visualization services
        • Cellular Translational Research Core (CTRC)providing stem cell and molecular biology services to investigators
        • Center for Human ToxicologyProvides bioanalytical services to investigators
        • Clinical Services Core (CSC)provides assistance to investigators in the design, planning, budgeting, and performance of human subject research
        • Community Engagement Services Coresupport researchers and leaders in becoming full partners in research projects
        • Genetic Science Learning Center (GSLC)major resources on learning about genetics and other scientific related fields
        • Behavior Health Innovation and Dissemination Centerhigh-quality evidence-based treatment to all
        • Scalable Analytics & Informatics (USAI)provides support to research and operations groups
      • Freezer
        • Freezer Programs
        • Freezer Information
        • Walk In Box
      • Links
        • Science Exchange
        • Vice President for Research
      • Work Auth Forms
        • On-Campus
        • Off-Campus
        • How to (Video)

      Methods

      You are here:
      1. Home
      2. Electron Microscopy
      3. Methods
      Sample Preparation

      Optimized Negative Stain Video
      Negative Stain Reference
      Negative-Stain Protocol

      Cryo-EM Sample Prep Videos
      Preparing Cryogenic Specimens

      Instrument Operation

      Operating Tecnai (T12 and TF20) Updated July 3, 2014
      Operating JEOL JEM1400 Updated Aug 22, 2014
      Operating Hitachi 7100 Updated Nov 2, 2016
      Using Low Dose (FEI instruments)
      Inserting and Removing Cryoholder from Pumping Station Updated Sep 29, 2014
      Finding a Lost Beam in a TEM Updated July 3, 2014

      Processing Film

      Loading, Unloading, and Developing Film

      Electron Microscopy
      • Methods
      • Resources
      • EM Software
      • Basic Unix Commands
      • Publications
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