The Mutation Generation and Detection Core (MGD) Facility specializes in providing custom TALEN and Crispr-Cas9 DNA nucleases to induce targeted mutations in a genomic region of your interest. TALEN and Crispr-Cas9 DNA nucleases are a cutting edge technology for performing reverse genetic studies in multiple model systems, including, but not limited to Zebrafish, Drosophila, C. elegans, Mouse, and mammalian tissue culture.
The MGD Core also offers services to identify induced mutations using High Resolution Melt Analysis (HRMA). Our support includes hardware, reagents, and expert advice for optimizing and performing HRMA for your gene of interest. The MGD Core also offers Services for preforming custom HRMA analysis for mutation detection and genotyping. Please contact the Director of the MGD Core for details on this service.
CRISPR-RNP Transfection Workshop: July 27-28
Presented by the Mutation Generation and Detection Core, The Center for Iron and Heme Disorders, and The Center for Clinical and Translational Science
This Workshop will present two days of morning informational seminars dicussing the use of CRISPR-RNP technology in cell culture cells from design, optimization, and delivery. Along with a Keynote address by Dr. Dana Carroll on the ethical and societal issues of the use of CRISPR technologies. Informational seminars will be open to all researchers.
The afternoon wet lab portion will allow researchers to gain hands experience with the production of CRISPR-RNP complexes and their Nucleofection into cell culture cells. If you are interested in registering for the wet lab portion, please contact the Mutation Generation and Detection Core at email@example.com or 801-585-0662.
Thursday, July 27
Eccles Auditorium, EIHG
09:00-10:30AM Dr. Greg Alberts Background on Genome Editing
10:30-10:45AM Coffee Break
11:00-12:00PM Dr. Greg Alberts Issues in CRISPR-Cas Editing
12:00-1:00PM Break for Lunch
1:00-5:00PM Wet Lab Day 1 MGD Core Room 7470 EIHG Production of CRISPR-RNP
Friday, July 28
Eccles Auditorium, EIHG
09:00-10:00AM Michael Collingwood “Mitigating risk of off-target effects when using CRISPR genome editing”
10:00-10:10AM Coffee Break
10:15-11:15AM Dr. Dana Carroll “Ethical and Societal Issues Surrounding Genome Editing”
11:30-12:30PM Break for Lunch
12:30-4:30PM Wet Lab Day 2 MGD Core Room 7470 EIHG Analysis of Nucleofected cells for