The Fragment Analyzer system is intended for quantifying and qualifying checks of DNA, gDNA, and RNA samples.  The system runs various kits measuring a range of fragment sizes from small PCR amplicons to very large DNA (10bp – 40,000bp).  The system has been adopted and incorporated into many current workflows, including for Illumina and PacBio NGS systems for library QC and input sample validation.

 

The system detects nucleic acids by using an intercalating dye and then running the samples out on a gel matrix in a very similar fashion to a capillary sequencer.  Results can be used to determine DNA or RNA quality before moving to sensitive downstream applications like NGS libraries, array runs, etc.

 

Results are delivered to the customer in the form of a PDF report from the manufacturer’s ProSize software.

 

More information about the Fragement Analyzer is available on AATI’s website here:  https://www.aati-us.com/instruments/fragment-analyzer/

 

Costs per run can vary by kit, but will be very similar.  One run is 1-11 samples plus a ladder.  The instrument runs all 12 positions whether or not there is sample.  Input amounts vary quite a bit kit by kit.  Please contact the core to discuss sample concentration.  We will use 2ul of the appropriate concentration per sample.  The kits currently stocked by the core:

 

High Sensitivity NGS Fragment Analysis kit
Internal U of U           External Academic or Government             Commercial

$85                                          $128.35                                              $170

 

High Sensitivity RNA Analysis kit

Internal U of U           External Academic or Government             Commercial

$85                                          $128.35                                              $170