The DNA Sequencing Core offers Next Generation Sequencing on the Ion Torrent PGM using several validated methods.  The PGM us ideal for smaller regions such as gene sequencing, mitochondrial sequencing, bacterial genomes, and gene panels.  Runs vary in data output (20MB for small runs up to in excess of 1GB for larger runs) based upon the parameters requested by the client and other variables such as library quality.

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PGM Methods:

  • Full Human mtDNA sequencing (~$300/sample)
  • BAC sequencing (~$1350/sample)
  • Large Amplicon sequencing (Cost varies)
  • Small Amplicon sequencing (Cost varies)
  • Bacterial genome sequencing (~$1350/sample)
  • Ion Torrent Produced panels listed below (prices vary by panel)

Costs assume the core will make and QC your library.  All costs are subject to change and will vary up or down depending on factors such as if a prepared library is submitted instead of our core making the library, etc. All costs listed are for internal university customers. For external customers, costs will be higher per University policy requirements.

In addition, we are willing to work with you to bring other applications online on this platform.  For estimation purposes, here are some fixed consumable prices.  Labor will be extra and will vary depending on how much we will need to do to support your project.

  • 314 chip: $99
  • 316 chip: $299
  • 318 chip: $499
  • Initialization 200 bp reagents to run up to 2 chips: $300
  • Initialization for 300 bp or 400 bp read for 1 chip: $300
  • Templating kit reagents for 1 chip: $125
Ion Torrent Produced panels listed below (prices vary by panel):

Ion AmpliSeq Cancer Panel v2:  Single-tube preparation of amplicon libraries from genomic “hotspot” regions that frequently mutate in human cancer genes.  These high quality libraries are prepared rapidly by PCR with as little as 10 ng of FFPE DNA or genomic DNA.

Ion AmpliSeq Comprehensive Cancer Panel:  Encompassing over 50% of the Wellcome Trust Sanger Institute Cancer Gene Census, this is the most comprehensive cancer gene panel available.  With all-exon coverage of 409 genes, the Ion AmpliSeq™ Comprehensive Cancer Panel delivers fast, FFPE-compatible, target selection for a broad survey of key genes for semiconductor sequencing.

Ion AmpliSeq Lung and Colon Cancer Panel:  Analyzes hotspots and regions in

22 genes with coverage of over 500 mutations implicated in colon and lung cancer.  Covering the following

22 genes:  KRAS, EGFR, BRAF, PIK3CA, AKT1, ERBB2, PTEN, NRAS, STK11, MAP2K1, ALK, DDR2, CTNNB1, MET, TP53, SMAD4, FBXW7, FGFR3, NOTCH1, ERBB4 , FGFR1, FGFR2. 

IonAmpliSeq BRCA1 & BRCA2 Panel:  Utilizes 167 amplicons to analyze the coding regions of tumor suppressor genes BRCA1 and BRCA2.

Ion AmpliSeq Inherited Disease Panel:  10,000 primer pairs that target 300 genes involved in some of the most common Mendelian diseases, making it ideal for a broad survey of inherited disease genes.

 

There are other variables that are less constant project to project that will affect your overall cost.  If you have questions, please call or email Derek Warner at (801)-581-4736 or at dwarner@cores.utah.edu.