CZAR Zebrafish

The CZAR (Centralized Zebrafish Animal Resource) facility provides state-of-the-art systems for housing, breeding, and doing experiments with zebrafish, an emerging vertebrate model system. It comprises 5000 fish tanks and redundant circulating water systems, and houses a large number of wildtype and mutant fish strains. It allows large genetic screens carried out as collaborations between multiple laboratories, and can provide animals and training for laboratories wishing to try pilot zebrafish experiments.

If you would like to make a reservation to use our equipment or services, please go to resource.cores.utah.edu.

The CZAR in the news:

http://www.sltrib.com/home/4919134-155/u-researchers-rely-on-federal-grants

Due to new NIH policy, NIH now uses an automated system to check all published Acknowledgements sections to determine what resources were used for the publication. This information is used to impact grant funding for the entire institution. Therefore, please be sure to acknowledge us, or any other Cores used for your publications, in the Acknowledgements section.

The cores are frequently called upon to validate their contributions to the University community and its research endeavors. One way we do that is through the number and quality of publications utilizing the cores. Your acknowledgements help us gain the recognition we need to continue to be supported by the University. Thank you for helping us so we can continue to serve you.

Example Acknowledgement Text (44 words): We would like to acknowledge the Centralized Zebrafish Animal Resource (CZAR) at the University of Utah for providing Zebrafish husbandry, laboratory space, and equipment to carry out portions of this research. Expansion of the CZAR is supported in part by NIH grant # 1G20OD018369-01

The CZAR is responsible for the daily care and maintenance of the fish and the aquatic systems. The Core provides six distinguishable services:

  1. It houses and maintains zebrafish, monitors their care, and establishes practices and provides oversight to ensure the safety and health of animals;
  2. It propagates several wildtype lines and provides animals from the lines to investigators;
  3. It provides laboratory bench space and supplies to perform experiments;
  4. It provides shared-use equipment including 7-8 microinjection stations, bright field stereomicroscopes, and a fluorescence stereomicroscope;
  5. It provides training to investigators; and
  6. It provides specialized centralized services performed by the permanent staff, such as sperm cryopreservation and storage.

Please contact the CZAR Director, Dr. Maurine Hobbs, if you have questions about the equipment or services.

Reserve Equipment for Use
Fees

Three types of fees for use of the Zebrafish Core Facility may be assessed:

  1. A ‘Laboratory User Fee’ is assessed to each laboratory that regularly uses the CZAR facility to cover maintenance of all equipment, disposable supplies, cleaning costs for matings, etc;
  2. Monthly per tank charges that cover upkeep of animals;
  3. Nominal fees to cover requests for sperm freezing and IVF services.

The precise fee schedule can be obtained from the CZAR Director.

Protocols

To help new users utilize the services of the fish facility, the following protocols are available for download in PDF or Excel format.

  1. Orientation and Access to CZAR 2016
  2. Setting Up Zebrafish to Spawn
  3. Instructions to Request Sperm Freezing
  4. Instuctions to Request IVF
Hours of Operation

Staff Support: Monday-Friday from 9am-5pm
Light Cycle: Sunday-Saturday from 9am-11pm

To help us ensure all fish experience the proper light cycle, please remember to turn off lights and close doors behind you after using any auxiliary rooms outside of the hours of Monday-Friday from 9am-5pm. Thank you.

Location

Due to certain circumstances, we are not able to disclose information about the CZAR’s location. Please call the Core lab at 801-585-6574 for our address and location.

Staff
MaurineHobbs-300x250

Maurine Hobbs, Ph.D., CZAR Director
Office: 801-585-1677
Lab: 801-585-6574
mhobbs@cores.utah.edu

SharonJohnson-300x250

Sharon Johnson, Senior Lab Specialist

Office: 801-585-1381
Lab: 801-585-6574
sjohnson@genetics.utah.edu

Talmage Long, Lab Assistant

Rotifer Room: 801-587-5808

Lab: 801-585-6574
talmage.long@cores.utah.edu

Oversight Committee

David Jonah Grunwald, Ph.D., 801-585-6421, grunwald@genetics.utah.edu
Joshua L. Bonkowski, Associate Professor of Neurobiology and Anatomy and Pediatrics
Richard Dorsky, Assoc. Professor of Neurobiology and Anatomy
David Jonah Grunwald, Professor of Human Genetics
Kristen M. Kwan, Assistant Professor of Department of Human Genetics
Amnon Schlegel, Asst. Professor of Internal Medicine; Member, Molecular Medicine Program
Rodney Stewart, Asst. Professor of Oncological Sciences
Jack Taylor, Director, Office of Comparative Medicine
H. Joseph Yost, Professor of Neurobiology and Anatomy and Pediatrics

Sexing Fish

For pictures and helpful tips on how to sex zebrafish, please visit:

http://www.zfic.org/common%20techniques/Gender%20identification%20guide.pdf

Embryonic Development

To find the original paper describing the various stages of embryonic development please visit:

http://homepages.wmich.edu/~dkane/Pubs/Kimmel%20staging%20series%2095.pdf

Wildtypes & Pigment Mutations

The CZAR maintains stocks of several different wild type and pigment mutants to facilitate our users research needs. Fish from a given line may be obtained either by requesting retiring brood stock, or by requesting extra embryos produced when the next generation of the line is made. Please contact Sharon Johnson at: sjohnson@genetics.utah.edu.

*AB (AB)
  • Pronounced star AB.

Male *AB (top) and Female *AB (bottom)

For more information on this line, please see the ZIRC web site at:

http://zfin.org/ZDB-GENO-960809-7

Tuebingen line

Male TU (top) and Female TU (bottom)

For more information on this line, please see the ZIRC web site at:
http://zfin.org/ZDB-GENO-990623-3

TL (Tupfel Long Fin)

Male TL (top) and Female TL (bottom)

For more information on this line, please see the ZIRC web site at:

http://zfin.org/ZDB-GENO-990623-2

WIK

Male WIK (top) and Female WIK (bottom)

For more information on this line, please see the ZIRC web site at:

http://zfin.org/ZDB-GENO-010531-2

Nacre
  • Homozygous nacre mutants lack melanophores throughout development but have increased numbers of iridophores.
  • This pigment mutant is due to mutations in the Mitfa gene.

Male Nacre (top) and Female Nacre (bottom)

For more information on this line, please see the ZIRC web site at:

http://zfin.org/ZDB-GENE-990910-11

Golden
  • Pigment mutant due to a mutation in the slc24a5 gene.

Male Golden (top) and Female Golden (bottom)

For more information on this line, please see the ZIRC web site at:

http://zfin.org/ZDB-GENE-031210-1