nikon_a1r

Equipment Type: Confocal Microscope
Location: Radiobiology Lab (Bldg 586) Room 56
U of U Personnel Rate: $20 Per Hour ($10 Nights and Weekends)
Reserve for Use

Main features of System:

  • Four Photo Multipliers Tube (PMT) detector unit enables simultaneous 12 bit (4096 intensity levels) acquisition of up to 4 fluorescent channels.
  • Fifth transmitted light detector available for simultaneous brightfield imaging of samples while scanning confocally.
  • Hybrid confocal scan head unit can provide high resolution images up to 4096 x 4096 pixels in size in conventional galvonometer mode and in high speed resonant mode up to 30 frames per second at 512 x 512 pixels.
  • Ultra high speed resonant acquisitions up to 420 frames per second at 512 x 32 pixels.
  • 32 channel spectral detector unit enables “Virtual Filter” and spectral demixing modes of confocal imaging.
  • Fully automated Nikon Ti-E inverted microscope.
  • Epifluorescence and DIC (Nomarski) illumination lamps for rapid sample identification and focusing.
  • Filter wheel used for epifluorescence viewing of sample is currently outfitted with with DAPI (blue), FITC (green), and TRITC (red), filter cube sets.
  • ASI MS-2000 Motorized XY stage with a variety of stage inserts available (slides,dishes, multiwall plates, etc..).
  • MCL Piezo Stage (100um range) for high speed 3D (i.e., Z-stack) volume acquisition.
  • Perfect Focus System provides focal depth consistency across varying sample regions of interest.
  • Nikon NIS-Elements multi-platform acquisition software.

Imaging lasers:

  • 405nm diode laser
  • 488nm Argon gas laser
  • 561nm Sapphire diode laser
  • 638nm diode laser

Objectives:

  • 4X
  • 10X
  • 20X
  • 40X oil
  • 60X oil
  • Other objectives available–please inquire with Core personnel