Leica SP8 White light Laser Confocal:

Location: HCI Room 1480

Schedule for Use

Main features of Leica SP8 White Light System:

  • Five detectors, Two Photo Multipliers Tube (PMT) detectors and three HyD detectors for enhanced sensitivity and dynamic range for 5 total fluorescent channels.
  • Transmitted light detector available for simultaneous brightfield imaging of samples while scanning confocally.
  • Hybrid confocal scan head unit can provide high resolution images up to 4096 x 4096 pixels in size in conventional galvonometer mode and in high speed resonant mode up to 30 frames per second at 512 x 512 pixels.
  • Ultra high speed resonant acquisitions up to 420 frames per second at 512 x 32 pixels.
  • Fully automated Leica DM8 microscope
  • Epifluorescence and DIC (Nomarski) illumination lamps for rapid sample identification and focusing.
  • Filter wheel used for epifluorescence viewing of sample is currently outfitted with with DAPI (blue), FITC (green), and TRITC (red), filter cube sets.
  • Laser autofocus system provides focal depth consistency across varying sample regions of interest.
  • OkoLab heating/cooling stage top incubator with C02 and humidity. Plates and dishes with glass bottoms.

Imaging lasers:

  • 405nm diode laser
  • 488nm Argon gas laser
  • White Light continuously tunable pulsed laser 440-700nm

Suggested Applications:

  • Multichannel fluorescence and transmitted fixed slide imaging
  • Tiling mosaics of fixed or live samples
  • Timelapse of dishes or glass bottom well plates

Objectives:

PL APO

  • 10X/0.40
  • 20X/0.75
  • 20X/0.75 Multi-Immersion
  • 40X/1.10 Water
  • 40X/1.30 oil
  • 63X/1.40 oil
  • Other objectives available–please inquire with Core personnel